2003年3月13日
就是這個鬼東西害我這麼"早"才可以睡.
為了這個報告, 我的電腦都快當機了, 開了20多個視窗, 豬!!!
不要問我我在寫什麼, 因為我自己都不知道.
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What I assigned is the sequence RRG6:
CAAATTCAAAGAAGAAGATGTACAGACTGCGGGCTAGAAACTGAAGAAATTGACATTTAA
Several DNA sequences are found over 65% homology with this specific fragment. However, only one DNA sequence is found 100% homologous with this sequence ---- RAD14 ---- found from position 665844 to 665903 on the Crick strand, chromosome XIII in baker's yeast in the BLAST test.
665844 5'-TTAAATGTCAATTTCTTCAGTTTCTAGCCCGCAGTCTGTACATCTTCTTCTTTGAATTTG-3' 665903
This gene functions as one of the genes that encodes for enzyme that repair damaged DNA binding activity, and involved in nucleotide excision repair system. The general pathway of nucleotide excision repair is similar in all organisms. A multiunit enzyme hydrolyzes 2 phosphodiester bonds on both sides of the DNA lesion. After the dual incision, the DNA lesion fragment is released from the duplex by DNA helicase, then the resulting gap is filled by DNA polymerase. The final nick will then be lygated by DNA lygase. RAD14 encodes one of the subunits of the repair complex, with which the DNA lesion fragment (pyrimidine(6-4)pyrimidone photoproducts) can be recognized then can be repaired.
This is not an essential gene. Systematic deletion of this gene will not affect primary metabolism of organism. This gene will only work under the condition when DNA is mutant, and need to be repaired by DNA repair mechanism. If there is no irritation that causes DNA mutation, then this gene will not be in use.
Xeroderma pigmentosum type A (XP-A) is an autosomal recessive human disease characterized by defective nucleotide excision repair and a severe predisposition to skin cancer. And yeast gene RAD14 is associated with this human disease.
1xpa and 1d4u (chain A)are proteins of known structure with sequence similarity to the Saccharomyces cerevisiae protein RAD14/YMR201C. They function as solution structure of the dna- and rpa-binding domain of the human repair factor xpa, nmr, 1 structure; and interactions of human nucleotide excision repair protein xpa with rpa70 and dna: chemical shift mapping and 15n nmr relaxation studies, respectively.
posted by Biochemie on 4:23 上午
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